Abstract
Neutrophils (PMN) play a key role in eliciting congenital immune response. These cells are equipped with specific receptors that are located on the surface of their cell membrane. These receptors produce various signals which in turn help in the effective functioning of PMN. The activity of these cells may be modified by factors of endo- and exogenous origin, including xenoestrogens such as bisphenol A (BPA). The aim of this study was to evaluate the effect of BPA on the expression of CD11c, CD14, CD15, CD16, CD62L and CD284 compounds on the surface of neutrophils in women and men. The study material included PMN isolated from the whole blood. The cells were incubated in the presence of BPA and/or LPS. Flow cytometry technique was used to evaluate the expression of CD antigens. Studies of these receptors indicate that BPA, at a concentration corresponding to the serum level of this compound in healthy subjects as well as at higher doses, induces changes in the immunophenotype of PMN, which may lead to immunity disorders associated with the dysfunction of these cells. Moreover, the observed effects of xenoestrogen on the expression of CD11c, CD14, CD15, CD16, CD62L and CD284 differentiation markers on these cells are sex-independent.
Highlights
Neutrophils (PMN) play a key role in eliciting congenital immune response
The expression of CD11c, CD14, CD15, CD16, and CD62L cell surface antigens was demonstrated on the cell membrane of PMN in women and men (Fig. 3)
Exposure of female PMN to bisphenol A (BPA) led to a decrease in the percentage of PMN expressing CD11c, CD15, and CD16 markers compared to cells not treated with xenoestrogen
Summary
Neutrophils (PMN) play a key role in eliciting congenital immune response These cells are equipped with specific receptors that are located on the surface of their cell membrane. These receptors produce various signals which in turn help in the effective functioning of PMN The activity of these cells may be modified by factors of endo- and exogenous origin, including xenoestrogens such as bisphenol A (BPA). Flow cytometry technique was used to evaluate the expression of CD antigens Studies of these receptors indicate that BPA, at a concentration corresponding to the serum level of this compound in healthy subjects as well as at higher doses, induces changes in the immunophenotype of PMN, which may lead to immunity disorders associated with the dysfunction of these cells. The receptors respond to the external stimuli by providing appropriate signals that further help in the activation and functioning of various intracellular transmission pathways, which include pathogen recognition, opsonization, phagocytosis, complement activation, and initiation of PMN death through apoptosis[15,16,17,18]
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