Effect of biotreatment with Thiobacillus ferrooxidans on the floatability of sphalerite and galena

Abstract

The bacterium Thiobacillus ferrooxidans triggers the surface modification of sulfide minerals to provide hydrophilicity or hydrophobicity for the flotation of sphalerite and galena. The effects of initial cell concentration, period of biotreatment and pulp density on the floatability of the minerals are discussed. With galena, flotation enhancement obtained through sulfuric acid treatment was retarded by biotreatment at all cell concentrations due to chemical effects. Such a decrease occurred for sphalerite only at cell concentrations sufficiently high for bacterial attachment to the mineral surface. The variables affecting surface modification (and, thus, flotation response of the minerals) include the cell concentration used for bacterial conditioning, the period of biotreatment and the pulp density. Although bacterial growth during biotreatment was observed even in the absence of nutrients, such growth was more common in the sphalerite suspension than in the galena suspension. Cell protein estimations suggest that more cells are attached on galena than on sphalerite. Probable reaction mechanisms associated with bacterial modification are discussed.