Abstract
In this study, biotin functionalized bovine serum albumin (BSA-biotin) loaded with curcumin (CUR), was synthesized and evaluated as drug carrier. The aim of this study is evaluation of the curcumin induced apoptosis related proteins, Bcl-2 and Bax expression via targeted albumin based nanoparticles. The synthesized BSA-biotin-CUR was characterized by different techniques such as: TEM, UV–Vis, FTIR, and DLS. The loading efficiency of CUR was 7.9%. The result indicates that the particles were measured around 185.4 nm with a zeta potential of -16.8 mV. Cytotoxicity results on mouse mammary tumor cell line (4T1) confirmed that formulated CUR, BSA-biotin-CUR, has high anticancer effect compared to free CUR. Also, real-time PCR method revealed that BSA-biotin-CUR increased Bax protein expression, and also downregulated Bcl-2 protein in 4T1 cells, which proven the ability of synthesized nanoparticles. We suggest that BSA-biotin-CUR can improve therapeutic effect, of CUR so that it can overcome the limitations of CUR with a great potential for further evaluations and applications.
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