Effect of biotic elicitors on secondary metabolite production in cell suspensions of Hypericum triquetrifolium Turra.

Abstract

Many experiments were designed to induce and increase the production of some secondary metabolites in tissue cultures of Hypericum triquetrifolium Turra using some biotechnological approaches. Callus was initiated on leaf discs cultured on MS medium supplemented with thidazirion (TDZ) at the concentrations 1.0, 1.25, 1.5, 2.0, or 2.5 mgL -1 and indole-3-acetic acid (IAA) at 0.5 mgL -1 ; callus was also initiated on stem explants on MS medium supplemented with 1.25 mgL -1 6-benzyl-aminopurine (BAP) and 0.5 mgL -1 of IAA. HPLC was used to determine the type and quantity of secondary metabolites in comparison with standards. Phytochemical accumulation in suspension cultures derived from leaf (LCs), stem (SCs) and root (RCs) were investigated. Fungal extracts of Aspergillus niger , Fusarium oxysporum and commercial yeast were added to a liquid MS medium at 0.1, 0.25, 0.5 or 0.75 mgL -1 . Data showed that the yield of p-OH-benzoic acid and chlorgenic acid in LCs treated with 0.5 mgL -1 yeast extract increased significantly compared with the control. Caffeic acid and tannic acid decreased in LCs significantly after elicitation with all biotic elicitors, but catechin accumulation in LCs increased significantly, when A. niger extract was added at all concentrations. Rutin, hypersoid and quercitin production in SCs increased significantly when treated with the fungal elicitors ; A. niger , F. oxysporum and yeast extracts. Chlorgenic decreased significantly in SCs, after the addition of all biotic elicitors at different concentrations.