Effect of Bioplastic Material on Adhesion, Growth and Proliferative Activity of Human Fibroblasts when Incubated in Solutions Mimic the Acidity of Wound an Acute and Chronic Inflammation

Abstract

INTRODUCTION. One of the key stages of wound healing is the phase of inflammation, which is a transitional process between hemostasis and wound healing. Each stage of the inflammatory-reparative process is characterized by its own value of the acidity of the wound bed. For example, in the acute stage of inflammation, the acidity of the medium in the wound bed decreases to a pH of 5.5-6. The chronic stage of the inflammatory process, on the contrary, is accompanied by an increase in pH to 8. To date, so far, the effect of biomaterials containing components of the intercellular matrix of the human dermis on fibroblasts under acidosis and alkalosis has not been fully investigated.
 AIM. To define the effect of bioplastic material based on collagen, hyaluronic acid and elastin on the viability and proliferative activity of human fibroblasts in conditions simulating the acidity of acute and chronic wounds.
 MATERIAL AND METHODS. Bioplastic material was made according to the method described in patent RU 2722744. Adhesive properties and proliferative activity of human fibroblasts were assessed visually using fluorescent microscopy. The number of apoptotic and dead cells was assessed by flow cytometry (BD FACSCanto II) using the commercial FITC Annexin V Apoptosis Detection Kit I (BD Pharmingen). The strength, Youngs modulus, and elasticity of the gels were determined on a TA.XT-plus texture analyzer (Stable Micro Systems, Great Britain).
 RESULTS AND DISCUSSION. Using the methods of luminescent microscopy and flow cytometry, we found that the cell viability (namely, adhesive properties and proliferative activity) decreases after incubation on condition mimic of physiological acidosis. We found that, bioplastic material contributes to the preservation of adhesive properties, viability and proliferative activity of fibroblasts in physiological acidosis conditions.
 CONCLUSION. The results obtained indicate that bioplastic material based on soluble dermis components can be used as a biologically active component of wound dressings for increase the effectiveness of reparative regeneration, especially in cases of excessive acute inflammation.