Effect of biglycan on apoptosis of neurons in acute cerebral infarction in rats

Abstract

ObjectiveTo investigate the effect and mechanism of biglycan (BGN) on apoptosis after experimental acute cerebral infarction (CI) in rat.MethodsA total of 110 clean grade Wistar male rats were randomly divided as follow: A total of 80 clean grade Wistar male rats were randomly divided into sham group and CI group consisted of 3h, 6 h, 12h, 24h, 48h, 72h, and 7d (n=10). The expression of BGN in neuron and its time course were detected. A total of 30 clean grade Wistar male rats were randomly divided into sham group, sham + control lentivirus group, sham + BGN lentivirus group, CI + control lentivirus group, and CI + BGN lentivirus group (n=6). The silence efficiency of BGN and the effect of BGN on neuronal apoptosis and neurological score after CI were detected. CI model was induced by reforming longa method. Lentiviral vector was administered intracerebroventricularly 7 days before CI. The expression of protein and mRNA of BGN were detected by western blot and real‐time quantitative PCR (qRT‐PCR). Immunofluorescence was conducted to explore the expression of BGN on neuron. Neurological score was detected by the modified Garcia score, beam balance tests and Longa’s Five‐Point Pacification Low. The TUNEL was used to detect neuronal apoptosis.ResultsThe expression of protein and mRNA of BGN were peaked at 24h after CI (P<0.05). A majority of BGN were detected on neuron at 24 h after CI. Compared with CI + control lentivirus group, the modified Garcia score, beam balance tests and the Longa’s Five‐Point Pacification were significantly improved in CI + BGN lentivirus group (P<0.05), as well as the TUNEL‐positive neurons ratio was significantly decreased ( P<0.05).ConclusionInhibition of BGN can significantly reduce early neuronal apoptosis after CI, and attenuated neurological deficits.Support or Funding InformationThis study was supported by the National Natural Science Foundation Youth Fund (30600637), China Postdoctral Foundation (2014M561207, 2019T120195), the Shanxi Province Basic Research Program (2010021034‐4, 201601D011119), the Shanxi Scholarship Council of China (2011‐096 and Key Projects No.4), Department of Human Resources and Social Security of Shanxi province, The science and technology research projects of health department of Shanxi Province (2016D011119), The Teaching Reform Program of Shanxi Higher Education, The Education Reform Project of Postgraduate Students in Shanxi Province, Lvliang people’s hospital fund, The Education Reform Project of Shanxi Medical University.