Abstract
beta-Carotene has shown antioxidant and anti-inflammatory activities in various cells and tissues. Oxygen radicals may act as important regulators for the expression of inflammatory enzymes such as inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) in H. pylori-infected gastric epithelial cells. This study was to investigate whether H. pylori-induced expression of iNOS and COX-2 and their products were inhibited by treatment with beta-carotene in gastric epithelial AGS cells. H. pylori strain of HP99 (a Korean isolate from duodenal ulcer patient) was used to stimulate gastric epithelial AGS cells with or without treatment of beta-carotene (2, 10, 20μM). HP99 induced the production of reactive oxygen species (ROS) in companied with the expression of mRNA and protein for iNOS and COX-2 in AGS cells. beta-Carotene inhibited HP99-induced production of ROS and expression of mRNA and protein for iNOS and COX-2 in AGS cells dose-dependently. Products of iNOS and COX-2 such as NO2 and PGE2 were also reduced by treatment of beta-carotene. In conclusion, beta-carotene may be beneficial for HP99-induced gastric inflammation by suppressing ROS-mediated expression of inflammatory enzymes. (This study was supported by Brain Korea 21 Project, College of Human Ecology, Yonsei University)
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