Abstract
The effect of benzyladenine (BA) on the activation of rRNA gene transcription was studied in wheat (Triticum aestivum and T. urartu) as related to the phenomenon of nucleolar dominance and the changes in the extent of methylation of the intergenic spacers in the subgenome A. The method of polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) was used to analyze the fragments of rDNA promoter regions amplified with the primers designed to recognize the sites of DNA isolated from BA-treated seedlings of diploid T. urartu and hexaploid T. aestivum and desaminated with metabisulfite. The subsequent genomic bisulfite sequencing of the amplification products was used to evaluate the level of methylation/demethylation of the particular cytosine residues. BA diminished methylation of cytosine residues in rDNA promoter regions to the level, which was different in two wheat species; these data presume that the transcriptional activities of rRNA genes in various wheat subgenomes depend on the extent of their methylation.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
