Abstract
To compare the efficiency of North Carolina State University medium 23 (NCSU23) and Alpha Minimum Essential Medium (α-MEM) as a base medium, and to evaluate the effects of Anti-Müllerian Hormone (AMH) alone or in combination with Follicle Stimulating Hormone (FSH) on the in vitro development and steroid production of isolated porcine preantral follicles. Porcine secondary follicles were cultured in NCSU23 or α-MEM media for 4 days. Once α-MEM was determined to be the optimal culture medium, secondary follicles were cultured in α-MEM alone or supplemented with FSH (1.5 ng/mL), AMH (50 ng/mL) or the combination of the two hormones. Follicle development was evaluated by measuring follicular growth, morphology and hormone production. There was no difference between the media NCSU23 and α-MEM in terms of follicle survival and growth (P > 0.05). However, at day 2, the antrum formation rate tended to be (P < 0.074) higher in α-MEM than NCSU23. At day 4 of culture, the estradiol and progesterone secretion were higher in α-MEM than NCSU23 (P < 0.01), while the opposite was observed for testosterone (P < 0.01). The addition of AMH and/or FSH did not affect follicular survival and growth. Nevertheless, the secretion of estradiol and progesterone induced by FSH was reduced with AMH (P < 0.01). α-MEM is a more effective base medium than NCSU23 for the in vitro follicular development of pig preantral follicles and AMH reduces the steroid production induced by FSH.
Highlights
Advances in reproductive biotechnology have increased the demand for large quantities of fertilizable oocytes
There are investigations comparing the efficiency of culture media in pigs, such as the North Carolina State University medium 23 (NCSU23) and the Tissue Culture Medium 199 (TCM199; Mao et al, 2002; Pyoos, Maqhashu, Scholtz, & Nedambale, 2018), there are no studies comparing the effects of NCSU23 medium and the Alpha Minimum Essential Medium (α-MEM) on the in vitro development of pig preantral follicles under the same culture conditions
The present study compared the efficiency of base media i.e., α-MEM and NCSU23, under the same experimental conditions, and for the first time the effects of Anti-Müllerian Hormone (AMH) and Follicle Stimulating Hormone (FSH) on the in vitro development of isolated pig pre-antral follicles
Summary
Advances in reproductive biotechnology have increased the demand for large quantities of fertilizable oocytes. Research has focused on developing a preantral follicular culture system that can be used to study early folliculogenesis and to serve as a source of oocytes for in vitro embryo production (Figueiredo, Rodrigues, Silva, & Santos, 2011; Marino et al., 2019). There are investigations comparing the efficiency of culture media in pigs, such as the North Carolina State University medium 23 (NCSU23) and the Tissue Culture Medium 199 (TCM199; Mao et al, 2002; Pyoos, Maqhashu, Scholtz, & Nedambale, 2018), there are no studies comparing the effects of NCSU23 medium and the Alpha Minimum Essential Medium (α-MEM) on the in vitro development of pig preantral follicles under the same culture conditions
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
