Abstract
The effect of basal medium, explant size and density on shoot multiplication, growth, rooting and acclimatization of date palm cv. ‘16-bis’ was evaluated. Bud clusters of different sizes (2, 3, 4 and 5 buds per cluster) were cultured at density of 1, 2, 3 and 4 clusters on Murashige and Skoog medium (MS), woody plant medium (WPM) and Nitsch medium (NM) supplemented with 0.5 mg/L 2-naphthoxyacetic acid and 0.5 mg/L kinetin for three months (multiplication phase). Separated shoots of different sizes (
Highlights
Date palm (Phoenix dactylifera L.) is one of the most important fruit crops in the Middle East and North Africa
The bayoud disease caused by a filamentous imperfect fungus (Fusarium oxysporum f. sp. albedinis) (Quenzar et al, 2001) is by far the most dangerous disease affecting date palm plantations (Sedra and Lazrak, 2011)
We aimed to evaluate the effect of basal medium, explant size and density on shoots proliferation and growth of the Moroccan date palm cultivar resistant to bayoud ‘16-bis’, in order to optimize conditions for an efficient micropropagation and regeneration through organogenesis
Summary
Date palm (Phoenix dactylifera L.) is one of the most important fruit crops in the Middle East and North Africa. Its population has been reduced significantly because of a very serious disease called bayoud (Sedra, 2011). The bayoud disease caused by a filamentous imperfect fungus Albedinis) (Quenzar et al, 2001) is by far the most dangerous disease affecting date palm plantations (Sedra and Lazrak, 2011). In Morocco, the majority of the date palm groves are affected by this fungus (Quenzar et al, 2001). The only feasible method to combat bayoud disease and rehabilitate Moroccan palm groves is the selection, multiplication the diffusion of resistant cultivars (Sedra, 2011)
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