Abstract
Conventionally, cultivations of Nepenthes are conducted by using seeds, cutting, and filial separation. However, there are many obstacles come both from time and technical aspect. In vitro culture is an alternative way for cultivating N. ampullaria (Jack,). One of a technique of in vitro culture is micro cutting. BAP (6-benzyl amino purine) growth regulator could be added to optimize the growth of N. ampullaria microcutting. The purpose of this research was to determine the effect of BAP on the growth of Nepenthes microcutting. This research was done experimentally using Completely Randomized Design (CRD). BAP treatment consisted of 5 concentrations: 0; 0.5; 1; 1.5; 2 (ppm), each treatment were multiplied 4 times. The parameters observed were: a time of bud initiation, time of root initiation, total of leaves, total of new buds, total of roots, length of leave, length of root, and height of bud. The data obtained were analyzed with ANOVA (Analisis of Variance) and continued with 5% and 1% LSD (Least Significant Different) test. The result showed that addition of BAP affected the growth of N. ampullaria microcutting in total leaves, length of leave, and total of buds. LSD test proved that 0.57 ppm of BAP was optimal concentration to increase total buds, whit the value reached of 3.86. Here, we found that BAP can be utilized to enhanche N. ampullaria growth on in vitro culture. The benefit of this study is to conserve N. ampullaria in vitro using BAP at 0.57 ppm.
Highlights
Wahyuni et al (2017) has noted that plant tissue culture methods or in vitro culture can be used as an alternative solutions to increase productivity, such as micro-propagation
In vitro culture is the process of multiplying cells, tissues, organs, or protoplasts with sterile techniques (Nasir, 2002)
Nepenthes propagation through micro cutting techniques can be done by culturing stems or shoots (Devi et al, 2013)
Summary
Nepenthes are rare and unique ornamental plants that have pitcher on the ends of the leaves (Sari et al, 2015; Handayani & Astuti, 2005; Schulze et al, 1997). Wahyuni et al (2017) has noted that plant tissue culture methods or in vitro culture can be used as an alternative solutions to increase productivity, such as micro-propagation. One of the in vitro culture techniques is micro cuttings. Nepenthes propagation through micro cutting techniques can be done by culturing stems or shoots (Devi et al, 2013). No scientific repots were available on the effect of BAP addition on the growth of N. ampullaria in vito micro-cutting culture. The results of this research are expected to provide scientific information about the best technique of N. ampullaria propagation, as well as knowing the best concentration of BAP on the growth of N. ampullaria in vitro micro cuttings. The growing media in each tube was added with five different concentrations of BAP, they were 0.0; 0.5; 1.0; 1.5; and 2.0 ppm, respectively. The obtained data were analyzed using Analysis of Variance (ANOVA) or F test, with 95% and 99% confidence levels followed with the LSD test
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