Effect of bamboo vinegar as an antibiotic alternative on growth performance and fecal bacterial communities of weaned piglets

Abstract

The aim of the study was to investigate the effects of bamboo vinegar as an antibiotic alternative in the diet of weaned piglets on their growth performance and fecal bacterial communities. The compound composition of bamboo vinegar was analyzed by gas chromatography–mass spectrometry (GC–MS). One hundred and twenty weaned piglets (Duroc×Landrace×Yorkshire), with an average weight of 8.4kg, were randomly assigned to five treatments, with three pens per treatment. The diets included bamboo vinegar at levels of 0, 0.2, 0.4 or 0.8%, or antibiotics, and designated as control, BV2, BV4, BV8 and antibiotic, respectively. Feed intake and weight gain of pigs were recorded at the start and at the end of the feeding trial. At the end of the experiment, fecal samples of four pigs from each treatment were taken to analyze the fecal bacterial communities analyzed by using 16S rDNA-based techniques. Amplicons of the V6–V8 variable regions of bacterial 16S rDNA were analyzed by denaturing gradient gel electrophoresis. Thirty four peaks (compounds) were identified or characterized in acetic ether extract from bamboo vinegar. The main group from bamboo vinegar was phenolic compounds, ketone and furfural. Daily weight gain of the pigs in BV4 and antibiotic was significantly higher than pigs in the control group. No significant difference was observed in daily weight gain among pigs fed diet containing bamboo vinegar and antibiotics. There was no significant difference in feed intake and feed to gain ratio among different treatment. The serum glutathione peroxidase activity of pigs in BV2 or BV4 was significantly higher than that of pigs in antibiotics treatment (P<0.05). The pigs in BV2 had significantly higher serum glutamic–oxaloacetic transaminase activity than those in control (P<0.05). No significant differences were found in serum superoxide dismutase, hydrogen peroxide, hydrogen peroxidase, oxidation resistance, malondialdehyde and glutamicpyruvic transaminase activities among different treatments (P>0.05). The richness and Shannon index of diversity were significantly lower for the pigs on the diet containing antibiotics than that of control or diets containing 0.2 or 0.4% bamboo vinegar, and tended to decrease with the increase of bamboo vinegar inclusion in the diets. The results demonstrate that bamboo vinegar in feed exerts an impact on the fecal bacterial community of piglets. The reasonable inclusion of bamboo vinegar, like antibiotics in piglet diet benefited for a better performance of piglets in this experiment. The result suggested that bamboo vinegar could be used as a potential additive in animal production as antibiotic alternative.