Abstract
Antilisterial bacteriocin producing strain were isolated from milk samples and were subjected to 16S rRNA sequencing and found to be of genus Enterococcus faecium. The bacteriocin ALC102 were partially purified by Amberlite XAD-16 adsorption followed by column chromatography. The biofilm formation capacity of Listeria monocytogenes MTCC 657 were evaluated by tube method and CV binding assay. Biofilm formation on different abiotic substrates were also evaluated. Among three substrates stainless steel had a strong biofilm formation followed by glass and aluminum foil. From the results of biofilm eradication studies, the bacteriocin ALC102 showed almost similar activity of commercial bacteriocin nisin on all the substrates at 45°C, 30°C, 4°C and -20°C. Based on CBD® biofilm eradication assay, the eradication potential of ALC102 and nisin were found to be similar on high (45°C) and freezing (-20°C) temperatures. From the study, antilisterial bacteriocin ALC102 found to be able to inhibit the biofilm formed Listeria monocytogenes MTCC 657 at different temperatures and different incubation periods (24h, 48h and 72h). The biofilm eradication potential of antilisterial bacteriocin ALC102 was similar to nisin. Neither incubation temperature nor incubation period doesn’t altered the activity of the bacteriocin. So this bacteriocin can be considered as a potential competitor in food industry and we strongly recommend the use of this bacteriocin from Enterococcus faecium GRD AA in the food preservation industry to a higher temperature (45°C) to freezing temperature (-20°C).
Highlights
Biofilms formed by microorganisms are often explained as immobile microbial consortia fixed in a three dimensional structure which consists of multicellular groups of prokaryotic and/or eukaryotic cells lodged in a matrix composed of not less than of matter incorporated by the microbial group.[1]
The sequence was submitted to GenBank, NCBI and got accession number MH113816 and the GRD S1 was named as Enterococcus faecium GRD AA
In this study, antilisterial bacteriocin production and biofilm eradication potential of Enterococcus faecium GRD AA isolated from milk sample were investigated in vitro
Summary
Biofilms formed by microorganisms are often explained as immobile microbial consortia fixed in a three dimensional structure which consists of multicellular groups of prokaryotic and/or eukaryotic cells lodged in a matrix composed of not less than of matter incorporated by the microbial group.[1]. Studies reported that biofilm is a well-established by the majority of the microorganisms in the environment exists as adhered to the surfaces as biofilm ecosphere as not as hovering microrganisms.[3]. Listeria monocytogenes is a Gram positive foodborne pathogen, which was first reported in 1926 in the course of an outbreak that affected rabbits and guinea pigs.[4] It was accepted as an etiological agent of human infections in the 1970s and recognized in 1980s5 as a foodborne pathogen. The bacteria are able to grow in pH range of 4.6 and 9.5 and has a low water activity of 0.92.8 Listeria discovers beneficial growth conditions on grounds, sewers and equipment in the food processing industry and environments preferably in the freezing and moist atmosphere of deep freeze rooms where non-psychotropic microbes commonly get through.[9,10]
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