Effect of Bacillus polymyxa as Wild or Mutant by UV and Diagnosed with PCR on the Available Phosphate Fertilizer in Calcareous Soils and Growth Yield of Corn (Zea mays L.)

Abstract

An agricultural experiment was conducted to study the effect of corn seeds inoculation with mutant, wild or mixed of them together of locally isolate of Bacillus polymyxa on the availability of phosphorus, growth and yield of corn (Zea mays L.) when it was planted in silty clay and loamy sand soils with the addition of four levels of triplsuperphosphate fertilizer (0, 50, 75 and 100 kg p/ha). B. polymyxa isolate was exposed to UV rays for 60 minutes to obtain mutagenic bacteria. Results showed that inoculation with wild isolate with 75 or 100 kg p/ha gave the best value for plant height, shoot dry weight, phosphorus concentration in the vegetative part and available phosphorus in the soil after planting. Inoculation with mutagenic isolate bacteria gave the value of 69.37 cm, 6 g/pot, 43.49 mg/pot and 27.52 mg/kg soil for plant height, shoot dry weight, phosphorus absorbed in vegetative part and available phosphorus, respectively when it planted in loamy sand or silty clay soil. On the other hand, the percentage of increase of the effect of phosphate fertilization levels was 47.82%, 39.70%, 75.53% and 73.46% in loamy sand soil and 46.74%, 56.96%, 85.33% and 53.12% in silty clay soil, respectively.