Effect of BA Treatments on Morphology and Physiology of Proliferated Shoots of &amp;lt;i&amp;gt;Bambusa vulgaris&amp;lt;/i&amp;gt; Schrad. Ex Wendl in Temporary Immersion

Yudith García-Ramírez,Ortelio Hurtado Ribalta,Marisol Freire Seijo,Leonardo Julio Moreno-Bermúdez,Mariana La O Cárdenas,Mallelyn Gonzales Gonzáles,Elisa Quiala Mendoza

doi:10.4236/ajps.2014.52027

Effect of BA Treatments on Morphology and Physiology of Proliferated Shoots of &lt;i&gt;Bambusa vulgaris&lt;/i&gt; Schrad. Ex Wendl in Temporary Immersion

Yudith García-Ramírez, Ortelio Hurtado Ribalta + Show 5 more

Open Access

https://doi.org/10.4236/ajps.2014.52027

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Abstract

Axillary buds, collected from greenhouse-grown plants of Bambusa vulgaris Schrad. ex Wendl (B. vulgaris), were incubated on a static liquid culture medium, Murashige and Skoog (MS) medium with 2% (w/v) sucrose, and supplemented with 12.0 μM 6-benzyladenine (BA). They were transferred to a temporary immersion system (TIS) using liquid MS medium supplemented with 0 (CK-free medium), 6.0, 12.0, 18.0 μM BA. The morphological and anatomical indicators were measured. The BA influenced in vitro multiplication of B. vulgaris. The best results were achieved in the SIT with a concentration of 6.0 μM of BA, which increased the number of shoots (5.1 shoots/explant) in the absence of hyperhydric shoots. Results demonstrated that the water content in the sprouts increased with 12.0 and 18.0 μM BA every four hours. Furthermore, these high levels of BA contributed to a lower accumulation of phenolic compounds and lignin content. The total chlorophyll significantly increased when using 6.0 uM BA, but decreased both parameters with other treatments. These results favor to increase the number of shoots/explants during in vitro multiplication. They will also optimize the in vitro culture conditions, leading to an improvement of in vitro propagation methods for this species.

Highlights

B. vulgaris (Bambusa vulgaris Schrad. ex Wendl) is considered within the genus Bambusa, the most important species globally
A mean of 4.90 normal shoots (NS) per explant was recorded in the temporary immersion system (TIS) plus 12.0 μM BA treatment, whereas a mean of 2.70 NS per explant was recorded in the static liquid culture medium at the same BA concentration (12.0 μM) (Table 1)
160 a b d c c basal Murashige and Skoog (MS) static liquid culture medium supplemented with BA and TDZ

Summary

Introduction

B. vulgaris (Bambusa vulgaris Schrad. ex Wendl) is considered within the genus Bambusa, the most important species globally. Ex Wendl) is considered within the genus Bambusa, the most important species globally. Tissue culture is the most commercially feasible method to produce bamboo plants that are as uniform as possible on a large scale and within a short space of time [1]. It is well known that mass propagation of plants by tissue culture in conventional semi-solid media is labor intensive and costly. Gelling agents contribute significantly to in vitro production costs and limit the possibility of automation for commercial mass propagation. New studies on in vitro propagation using different culture conditions can contribute to further optimization of the process and to reducing production costs [6]. Using liquid media in micropropagation processes is considered the ideal solution for reducing plantlet production costs and enabling automation [7]

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