Abstract
Fractionation technologies that produce higher value coproducts from dry grind corn processing have been developed. In one of the fractionation technologies, dry degerm defiber (3D) process, separation of germ and pericarp fiber is performed prior to fermentation of the endosperm fraction. Fermentation characteristics of endosperm fraction from the 3D process are inferior to ground whole corn because of a deficiency in lipids essential for yeast growth. Lipid supplementation and B vitamins separately improved glucose utilization and increased ethanol concentration. In this study, combinations of B vitamins and lipids were used to evaluate synergistic effect of two micronutrients added during the fermentation of endosperm fraction from 3D process. Combinations of B vitamins and lipids enhanced ethanol production by improving glucose utilization (due to B vitamins) and higher ethanol tolerance (due to lipid supplements). All lipid and B vitamins treatments had higher final ethanol concentrations compared to control with no added supplements. One of the six lipid supplements tested showed higher ethanol concentrations compared to other lipid supplements. Higher fermenter productivity and higher final ethanol concentrations can be achieved by lipid and B vitamin supplementation of endosperm fraction from 3D process.
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