Effect of Aurora-A inhibitor 1 on proliferation, apoptosis of PANC-1 cells

Abstract

Objective To investigate the expression of Aurora kinase A in normal human pancreatic epithelial cell and pancreatic cancer cell lines, and explore the role of Aurora-A inhibitor 1 on proliferation, apoptosis of pancreatic cancer cell PANC-1. Methods The expression level of Aurora-A and p-Aurora-A protein in two different cells was detected using Western blotting. Cell counting kit-8 (CCK-8) was constructed to investigate cell proliferation. Flow cytometry (FCM) and Western blotting were used to detect cell cycle and cell apoptosis. Results Western blotting indicated that Aurora-A and p-Aurora-A were over-expression in pancreatic cancer. The results of CCK-8 showed that Aurora-A could inhibit the proliferation of PANC-1 (P=0.019). The test of FCM showed that Aurora-A inhibitor arrest the cells during G2/M [Blank: (9.6±2.3)%, dimethyl sulfoxide (DMSO): (7.5±4.8)%, Aurora-A inhibitor 1: (61.2±4.0)%] and Western blotting further showed p21 was up-regulated (Blank: 1.000±0.000, DMSO: 1.671±0.111, Aurora-A inhibitor 1: 3.208±0.284). Moreover, FCM indicated that the apoptosis was significantly increased in Aurora-A inhibitor 1 group after treatment (Blank: 1.000±0.000, DMSO: 1.053±0.108, Aurora-A inhibitor 1: 1.902±0.276). The results of Western blotting showed the expression of Cleavage of cysteinyl aspartate specific protease (Cleaved Caspase-3) was raised (Blank: 1.000±0.000, DMSO: 1.274±0.112, Aurora-A inhibitor 1: 1.754±0.062). Conclusion Aurora-A and p-Aurora-A is over-expression in pancreatic cancer. Inhibition of p-Aurora-A by Aurora-A inhibitor 1 can arrest pancreatic cancer cell in G2/M. Moreover, Aurora-A inhibitor 1 decrease proliferation and promote cell apoptosis. Key words: Pancreatic cancer; Aurora-A; Inhibitors; Proliferation; Apoptosis