Abstract
为研究生长环境中微生物对铜绿微囊藻碳代谢的影响,本文分析太湖典型微囊藻水华样品附生菌中产碳酸酐酶(CA)细菌的比例,结果显示CA菌占11.6%;从微囊藻群体中分离获得了一株高胞外CA附生菌P201,通过ITS基因鉴定,该菌为一株荧光假单胞菌(Pseudomonas fluorescence).并研究了该菌在不同浓度HCO<sub>3</sub><sup>-</sup>条件下对铜绿微囊藻生长的影响,结果表明无论是高HCO<sub>3</sub><sup>-</sup>浓度还是低HCO<sub>3</sub><sup>-</sup>浓度环境中,加入该菌对铜绿微囊藻的生长均有促进作用,说明产CA酶附生菌对铜绿微囊藻的生长有一定的促进作用.;To study the effect of attached bacteria on the carbon metabolism of M. aeruginosa, the percentage of carbonic anhydrase(CA) produced bacteria in total attached bacteria was analyzed. The result showed that the ratio of CA produced bacteria is 11.6% in a typical cyanobacterial bloom from Lake Taihu. An attached bacterium strain P201, which produced large amount of extracellular carbonic anhydrase, was isolated from M. aeruginosa assemblage. It was identified as a strain of Pseudomonas fluorescence by its ITS molecular characteristic. Effects of P201 on the growth of M. aeruginosa in different HCO<sub>3</sub><sup>-</sup> concentrations were investigated. The results showed that no matter in the higher or the lower HCO<sub>3</sub><sup>-</sup> culture medium, P201 could promote the growth of M. aeruginosa, which suggested that CA produced attached bacteria could enhance the growth of M. aeruginosa by regulating the ambient carbon environment.
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