Effect of astroglial cell swelling on pH of acidic intracellular compartments

Abstract

A variety of pathological conditions lead to swelling of astrocytes, which in turn stimulates ion release by activation of ion channels at the plasma membrane. In the present study, acridine orange and fluorescein isothiocyanate coupled to dextran (FITC-dextran) have been used to examine the effect of cell swelling on pH in acidic compartments of cultured astroglial cells. Both NH 4Cl (2 mM) and chloroquine (10 μM), known to alkalinize acidic cellular compartments, led to the expected increase in acridine orange fluorescence intensity. Similar, albeit smaller, effects were elicited by a reduction of extracellular osmolarity (−80 mOsm) and treatment of the cells with glutamate (1 mM), manoeuvres which enhanced cell volume. Determination of changes in the FITC-dextran fluorescence ratio ( 485 440 nm ) allowed quantification of the pH changes in lysosomal compartments. Treatment with NH 4Cl, reduced extracellular osmolarity and glutamate increased lysosomal pH by 0.65 ± 0.07, 0.85 ± 0.14 and 0.25 ± 0.07, respectively. Measurement of cytosolic pH using 2′,7′-bis-(2-carboxyethyl)-5- (and -6) carboxyfluorescein (BCECF) demonstrated a pronounced acidification following cell swelling, observed with both reduced extracellular osmolarity (by 0.23 ± 0.05 pH units) and 1 mM glutamate (by 0.26 ± 0.02 pH units). In conclusion, pH within lysosomes and possibly other acidic cellular compartments of astrocytes is increased by cell swelling, which may have important consequences for astrocyte function.