Abstract
In this work, a water-soluble Astragalus membranaceus polysaccharide (AMP) was prepared by hot water extraction, and the effects of AMP on the serum cytokine levels and spermatogenesis of Kunming mice were investigated. Sixty Kunming mice were randomly divided into five groups: a normal control group, a model control group (treated with cyclophosphamide) and three treatment groups (treated with cyclophosphamide and 25, 50 and 75 mg/kg AMP). The effects of AMP on the serum cytokine levels and spermatogenesis of mice were evaluated. Intragastric treatment with different levels of AMP significantly increased serum interleukin-11, tumour necrosis factor-α and interferon-γ levels; protein expression and superoxide dismutase activity in testis; and sperm density, sperm movement and the rate of normal sperm morphology. In addition, AMP decreased the nitrate nitrogen level in the testes of Kunming mice compared with the model control group. The results indicated that AMP can ameliorate the immunity and spermatogenesis of mice with reproduction system impaired by cyclophosphamide.
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