Effect Of Astragalus Injection Combined With Aerobic Exercise On Myocardial Renin-angiotensin System In Diabetic Mice

Abstract

PURPOSE: This investigation aimed at investigating the synergistic effect of Astragalus injection combined with aerobic exercise on myocardial renin-angiotensin system in diabetic mice,and clarify whether the myocardial disease of diabetic mice is improved. METHODS: 32 6-week-aged male db/db mice were randomly divided into 4 groups,Diabetes mellitus group, Astragalus injection group,Swimming group and Astragalus injection + swimming group.Monitor the amount of drinking water,body weight and blood glucose changes.The myocardial tissue was executed after 6 weeks and the ventricular mass index was calculated;detect the angiotensin II (Ang-II),angiotensin-converting enzyme (ACE) and angiotensin converting enzyme 2(ACE2) in each group of myocardium tissues. RESULTS: In addition to diabetic group, the ventricular mass index of each group was elevated (P<0.05).In addition to the diabetic group, the Ang-IIlevels of myocardial tissues were decreased (P<0.05).In addition to the diabetic group, the ACE and ACE2 levels averaged increased (P<0.05). CONCLUSIONS: Myocardial ACE,ACE2 enzyme activity is generally low, Ang-II content is also higher than other groups, and the decrease of enzyme activity will promote the increase of Ang-II content, indicating that renin-angiotensin system is overactivated, resulting in strong vasoconstriction and myocardial ischemia and hypoxia, and eventually lead to all kinds of cardiomyopathy. After the intervention of astragalus membranaceus or aerobic exercise, the activity of ACE,ACE2 enzyme was increased and the level of Ang-II content was also significantly suppressed. Astragalus membranaceus or aerobic exercise had a positive effect on renin-angiotensin system in myocardial tissue of diabetic mice, and played a protective role in the myocardium of diabetic mice. It was speculated that there was a great deal of inhibition on renin-angiotensin system in myocardium of diabetic mice. It may be achieved by increasing the enzyme activity of ACE2 to inhibit the formation of Ang-II in diabetic myocardial tissue. FUNDING: This study was supported by Jiangsu Province Graduate Research and Innovation Program(KYCX18_1719)