Effect of ascorbic acid, sodium citrate, sodium bicarbonate, and their combination effect on the binding of 59Fe 2+ and 59Fe 3+ to plasma membrane of lactating mouse mammary gland

Abstract

Isolated plasma membranes of lactating mouse mammary gland were treated with different concentrations of ascorbate, sodium citrate, sodium bicarbonate, combinations of them (from 16 x 10 −10 to 4 x 10 −6 moles/L) and studied for the binding of 59Fe 2+ and 59Fe 3+ at pH 7.4. The results show that the Fe 3+ form of iron is under a greater influence of anions used in these experiments. The Fe 2+ form of iron is weakly bounded and affected. It is suggested that the form with a greater positive electric charge is more effectively bound to the receptors in plasma membranes.