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Abstract
Thioglucosidase activity was demonstrated in partially-purified preparations from several Cruciferae oilseeds, both in the presence and absence of ascorbic acid. The amount of activation by ascorbic acid differed among the enzyme preparations from different species. Buffer composition and pH were found to significantly affect enzyme activity, the turret rape enzyme showing a second optimum at pH 7·1 in the presence of ascorbic acid and sodium phosphate buffer. Disc electrophoresis on polyacrylamide gel revealed distinct isoenzyme patterns from crude extracts of all nine species or varieties studied. Some differences in the patterns were noted from electrophoresis of partially-purified preparations. Ascorbic acid was found to affect isoenzyme patterns and the rate of development of equivalent isoenzymes from yellow mustard and from turret rape.
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