Effect of artificially designed short peptide addition strategy on glucose oxidase activity

Abstract

Charged short peptides and glucose oxidase (GOx) were studied in this paper to reveal how these peptides alter the activity and structure of GOx. Results showed that the synthesized negatively charged peptides T8− and positively charged peptides T9+ could increase and decrease enzyme activity, respectively. The T8− treated GOx potential increased by 33.6%. Meanwhile, the α-helix content decreased by 5.5%, and the root mean square deviation (RMSD) value decreased significantly. The fluorescence and UV–visible spectra revealed varying degrees of changes in peak and peak intensity, respectively. Overall, in the absence of contact reactions between T9+ and T8− with GOx, the CPs changed the microenvironment around GOx and the charge arrangement on its surface, causing conformational changes in GOx and altering its enzymatic activity. The effect of CPs on GOx was further investigated using the molecular docking technique. After T8- treatment, the number of hydrogen bonds increased from 3 without short peptide treatment to 8