Effect of artesunate on the transplantation tumor of parotid gland in the nude mice with adenoid cystic carcinoma drug resistance cell

Abstract

Objective:To observe the effect of Artesunate on the transplantation tumor of parotid gland in the nude mice with adenoid cystic carcinoma drug resistance cell and to explore its clinical application prospect as an anticancer drug.Method:Twenty male BALB/C mice were chose to set up parotid gland transplantation tumor model, then used the method of random Numbers table 20 nude mice were randomly divided into normal saline control group, positive control group DDP 2 mg/kg group, the ART of 200 mg/kg group, ART 100 mg/kg+DDP 1 mg/kg group, a total of 4 groups. Abdominal cavity injection was performed twice a day, and we measured weight of each nude mice and transplantation tumor major axis and minor axis. Treatments were conducted for 4 weeks, then the mice were sacrificed, and tumor weight was measured to calculate the inhibitory rates. The tumors were examined by HE staining to observe each tumor tissue pathological changes and by immunohistochemical method to detect Survivin expression in transplanted tumor tissue. Result:Four weeks after treatments, the inhibitory rate of DDP 2 mg/kg group was 59.86%, but this group of nude mice lost weight obviously； the ART 200 mg/kg group was 43.54%，this group of nude mice had a good mental state, and weight had gaining continuously；the ART 100 mg/kg+DDP 1 mg/kg group was 41.16%, this group of nude mice had reducing weight slightly.HE staining showed:The tumor nuclei were hyperchromatic and shrinkage, nuclear chromatin condensed and cracked into flake in the ART 200 mg/kg group, DDP 2 mg/kg group and the ART 100 mg/kg+DDP 1 mg/kg group. Conclusion:Artesunate can inhibit adenoid cystic carcinoma resistant cells NACC/DDP proliferation in nude mice in vivo and promote its apoptosis, and the side effects of Artesunate on nude mice were lower than Cisplatin.