Abstract
Asparagus offi cinalis has been widely studied, but little information is available about its in vitro response to exogenous cytokinin during shoot multiplication. To study the effects of different cytokinins on shoot multiplication of A. offi cinalis ‘Grolim’, in vitro culture was initiated from shoot segments cultured on media with Murashige and Skoog medium. Effects of different aromatic cytokinins (6-benzylaminopurine, 6-benzylaminopurine riboside and meta-topolin) applied in four concentrations (0.5, 1.0, 1.5, 2.0 mg/l) on shoot multiplication of ‘Grolim’ were tested. Effect of explant position (vertically or horizontally) on the shoot multiplication outcome was also studied. Both the length and the number of newly developed shoots were signifi cantly affected by explant position and cytokinin content of the medium. The highest numbers of shoots (4.9) were produced in the presence of 0.5 mg l-1 6-benzylaminopurine riboside when explants were paced horizontally onto the medium. Although the longest shoots (41.5 mm) developed on explants placed vertically onto medium supplemented with 2.0 mg l-1 meta-topolin, the lengths of shoots developed on medium with 0.5 mg l-1 6-benzylaminopurine riboside were also adequate in both explant position (29.5 and 33.6 mm placed horizontally and vertically, respectively).
Highlights
Members of Asparagus genus have been cultivated for over 2000 years as a vegetable and medicinal herb (A. racemosus, A. verticillatus, A. adscendens and A. curillus)
Many researchers have investigated the possibilities of micropropagation of A. officinalis (Murashige et al, 1972; Yang & Clore, 1974; Yang, 1977) and found the Murashige and Skoog (1962) MS-based culture media to be optimal for asparagus
In the experiments of Sarabi & Almasi (2010) asparagus explants were grown on MS medium supplemented with 6 % sucrose and effects of 1-naphthaleneacetic acid (NAA) and 6-benzylaminopurine (BA) concentrations on callus induction and shoot development were tested
Summary
Members of Asparagus genus have been cultivated for over 2000 years as a vegetable and medicinal herb (A. racemosus, A. verticillatus, A. adscendens and A. curillus). Asparagus officinalis is a perennial, dioecious species with male and female plants, and a wellknown vegetable widely cultivated all over the temperate world (Desjardins, 1992). In the experiments of Sarabi & Almasi (2010) asparagus explants were grown on MS medium supplemented with 6 % sucrose and effects of 1-naphthaleneacetic acid (NAA) and 6-benzylaminopurine (BA) concentrations on callus induction and shoot development were tested. Their results showed that 0.015 mg/l NAA and 0.5 mg/l BA gave the highest number of plantlets
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