Abstract
Objective To investigate the effect of arctigenin on the apoptosis of FBL-3 cells and its mechanisms. Methods The mouse erythroleukemia FBL-3 cells were taken as subjects. The untreated FBL-3 parental cells were taken as the control group, and the FBL-3 cells treated with 20 mg/L arctigenin for 24 h were taken as the experimental group. The effects of arctigenin on the apoptosis of the mouse erythroleukemia FBL-3 cells were determined by agarose gel electrophoresis and flow cytometry assay. The changes of apoptosis-related genes were analyzed by reverse transcriptase-polymerase chain reaction(RT-PCR). Results The agarose gel electrophoresis results revealed that the DNA ladder was displayed in the experimental group compared to the control group. The flow cytometry data demonstrated that the apoptosis number of FBL-3 cells in the experimental group was markedly increased compared to that in the control group (t=60.681, P=0.000). The RT-PCR assay results suggested that the expressions of Bcl-2 and IAP-1 gene were down-regulated in the experimental group compared to the control group (t=14.732, 29.702, all P=0.000), while the expressions of Bax and Smac gene were up-regulated(t=6.721, 8.499; P=0.003, 0.001), but the expression of Bcl-XL did not change(t=0.209, P=0.844). Conclusions Arctigenin can induce the apoptosis of the mouse erythrolenkemia FBL-3 cells. Apoptosis of the mouse erythrolenkemia FBL-3 cells induced by arctigenin may be correlated to the down-regulation of Bcl-2 and IAP-1 and up-regulation of Bax as well as Smac. Key words: Arctigenin; Leukemia; Apoptosis; Anti-apoptosis gene; Pro-apoptosis gene
Published Version
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