Abstract
The present study is aimed to investigate the influence of an aqueous extract of Tribulus terrestris on some biochemical parameters namely, alkaline phosphatase (ALP), Iron, phosphate (PO4), sodium (Na+), potassium (K+), aldosterone, and cortico steroid in unilateral and bilateral castrated male rabbits. Thirty mature male rabbits which are divided randomly in to six groups. group 1: is control, group2: orally administrated 150 mg / kg / day of an aqueous extract of T. terrestris, group3: is bilateral castrated rabbits received D. W., group4: is bilateral castrated rabbits received (150 mg / kg / day) of the extract, group 5: unilateral castrated rabbits received D. W., group 6 is unilateral castrated rabbits received 150 mg / kg / day of the extract, all doses were given orally every day for 30 days. At the end of the experiment, sera were collected and biochemical tests measured. The results showed a significant (P<0.05) increased in ALP level in group 2 and in PO4 level in group 5 in comparison with group1, while PO4 level tended to be decreased significantly (P<0.05) in group 6 when it compared with group 5. The results also showed a significant(P<0.05) increased in (K+) and (Na+) in group 2 and group 6 in comparison with group1 while there was a significant(P<0.05) decreased in (K+) and (Na+) in group 4 when it compared with group 2. In addition there was a significant (P<0.05) increased in aldosterone concentration in group 6 compared with group 1 and group 2. While there was a significant decreased in cortico steroid concentration in group 3 , 5 and 2 compared with group 1 but the cortico steroid showed a significant (P<0.05) increased in group 6 compared with group 1. There were no significant differences between groups concerning the iron concentration.
Highlights
The requirements of the present market for ecological production laid the use of plant extracts in the feed industry as an alternative nutritive antibiotics and synthetic hormones (1)
Preparation of an aqueous extract for T. terrestris fruits was carried out as follows, 100 gm of dried fruits was transferred in to a conical flask and 500 ml of distilled water was in a rate of 1:5 w:v shaked by electric shaker for 2 hours and left in room temperature for 24 hours, the mixture was filtered by four layers of gauze and put in plan tube and centrifuged mixture in centrifuge at 2000 rpm for 10 minute
The results of the analysis showed a significant (P
Summary
The requirements of the present market for ecological production laid the use of plant extracts in the feed industry as an alternative nutritive antibiotics and synthetic hormones (1). One of the most popular phytoproducts is an extract of annual herb Tribulus terrestris L. The occurrence of glycosides, steroids, saponins, flavonoides, alkaloids, tannins, vitamins, unsaturated fatty acid, cinammic acid, and amides has been reported in Tribulus terrestris (2). This plant has been used in the folk medicine by Arabs (3) and it has played an important role as aphrodisiac plant in east countries and Bulgaria, and for treatment of erectile dysfunction, diabetes, tumors, cardiovascular-, respiratory-, and other diseases (4). The objectives of this study are to examine the effect of aqueous extract of T. terrestris on some biochemical parameters of blood serum in normal and unilateral, bilateral castrated rabbits There are many clinical investigations about the effect T. terrestris on humans and labor animals were carried out, but there are no data concerning the effect of T. terrestris extract on Iron.
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