Effect of aqueous extract of the leaves of Baccharis trimera on the proliferation of hepatocytes after partial hepatectomy in rats.

Sonia Oliveira Lima,Danillo Primo Andrade Santana,Juliana Cordeiro Cardoso,Erick Sobral Porto,Maria Bernadete Galrão De Almeida Figueiredo,Matheus De Souza Nogueira,Ricardo Luiz Cavalcanti De Albuquerque Junior,Vanessa Rocha De Santana,Renata Lima Batalha De Andrade,Jessica Macêdo Santos

doi:10.1590/s0102-865020170040000001

Sonia Oliveira Lima, Danillo Primo Andrade Santana + Show 8 more

Open Access

https://doi.org/10.1590/s0102-865020170040000001

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Abstract

To evaluate the effect of aqueous extract of Baccharis trimera leaves on the proliferative capacity of the liver after partial hepatectomy (PH) in rats. Twenty Wistar rats weighing between 300 and 450g were divided into two groups: control (HP) and test (HP100-rats that received the aqueous extract of Baccharis trimera for four days at a dose of 100 mg / kg / day). On the fifth day, animals from both groups underwent resection of 70% of the liver. Twenty-four hours later, they were sacrificed and the remnant liver was removed and prepared for studied through PCNA immunohistochemistry. Data analysis for comparison between the two groups was made through the non-parametric statistical test Mann-Whitney test. In all the animals studied was found most abundant nuclear immunostaining positive hepatocytes interlobular located in regions of the liver. Quantitative analysis of PCNA-positive cells revealed positivity rate significantly higher mean (p = 0.02) in HP100 group (77.1 ± 13.6) compared to the HP group (45.8 ± 12.9). DAdministration of aqueous extract of the leaves of Baccharis trimera 100 mg/kg of animal has a significant positive effect on liver regeneration in rats, 24 hours after hepatectomy (70%).

Highlights

According to data from the World Health Organization (WHO), about 80% of the world’s population uses medicinal plants, especially those from developing countries, and the lowincome population is the largest consumer[1]
Brazil has an extremely rich flora and a tradition in the use of medicinal plants, most of the time, this is based only on popular knowledge, without any pre-clinical or clinical evidence[4]. In this context, knowing that the liver is an organ with high proliferative capacity that restores the lost hepatic mass through a global hyperplasia of the remaining parenchyma[5], there is a great variety of substances tried in partially hepatectomized animals, in which evaluates their performances in the regenerative process[6]
Due to the large number of liver diseases, the popular use of the carqueja and the lack of scientific studies on the effect of this plant on liver regeneration, the objective of this study was to evaluate the effect of the aqueous extracts of Baccharis trimera leaves on the proliferative capacity of the liver after extended hepatectomy in rats

Summary

Introduction

According to data from the World Health Organization (WHO), about 80% of the world’s population uses medicinal plants, especially those from developing countries, and the lowincome population is the largest consumer[1]. Brazil has an extremely rich flora and a tradition in the use of medicinal plants, most of the time, this is based only on popular knowledge, without any pre-clinical or clinical evidence[4]. In this context, knowing that the liver is an organ with high proliferative capacity that restores the lost hepatic mass through a global hyperplasia of the remaining parenchyma[5], there is a great variety of substances tried in partially hepatectomized animals, in which evaluates their performances in the regenerative process[6]. The herbal products in nature appear as an important segment in this search[7]

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