Effect of apatite-wollastonite glass ceramics on the migration of mesenchymal stem cells

Abstract

Event Abstract Back to Event Effect of apatite-wollastonite glass ceramics on the migration of mesenchymal stem cells Sylvia Muller1, Lindsey Nicholson1, Julian De Havilland1, Kenny Dalgarno1, Anne Dickinson1 and Xiao-Nong Wang1 1 Newcastle University, Institute of Cellular Medicine, United Kingdom Osteoarthritis is a progressive degenerative disease which results in the destruction of the bone and cartilage of the joints, primarily in the hands, hips and knees. Alterations in bone homeostasis leaves bones brittle and susceptible to damage while an increased production of enzymes reduces the structural integrity of the cartilage matrix, leaving it easily eroded. Apatite-wollastonite glass ceramic (A-W) is a biocompatible and osteoconductive material which can be formed into a tissue scaffold with the use of a 3D-printer. Osteogenic cells such as mesenchymal stem cells (MSCs) can be grown on these scaffolds in vitro and will undergo osteogenic differentiation. Additionally, resident MSCs in the areas surrounding the site of implantation may be able to migrate towards the scaffold to add to the bone formation. MSCs are traditionally isolated from sources such as the bone marrow by their adherence to plastic. The population this yields is often highly heterogeneous in phenotype and functional properties. Techniques for the enrichment of cell population based on marker expression have made it possible to generate a cell population with greater osteogenic potential. CD271+ MSCs, thought to be progenitor MSCs, have been shown to be osteogenicly primed and hence may be better suited for bone regeneration. This study compared the migrational potential of MSCs derived from plastic adherent cells (PA-MSCs) and MSCs derived from a population enriched for CD271highCD45- cells (CD271-MSCs). The aim of the work was to assess if A-W scaffold conditioned media could induce the migration of the two fractions of MSCs. This was done using cell or A-W scaffold conditioned media in a chemotaxis assay using multi-chambered slides and 18 hour time-lapse video microscopy. The known chemoattractant CXCL12 was used as a positive control and standard culture media was used as a negative control. Our results revealed that neither fraction of MSC had directed migration towards scaffold conditioned media. Additionally, A-W scaffold conditioned media was seen to inhibit MSC migration towards a known chemo-attractant. PA- and CD271-MSCs had similar patterns of migration towards the positive control CXCL12. PA-MSCs conditioned media was a strong chemo-attractant for PA-MSCs; however CD271-MSC conditioned media had little to effect on the migration of CD271-MSCs. Overall, this work has shown that A-W scaffolds may have an inhibitory affect on the potential of cells to migrate, which may have a negative impact on the overall quality of scaffold based regeneration. Additionally, the work suggests that the production of chemo-attractants from PA-MSCs and from CD271-MSCs is different. This work can help towards the greater understanding of MSC migration and homing. This work has been funded by Arthritis Reseach UK (ARUK) Keywords: Bone Regeneration, stem cell, Scaffold, Tissue Regeneration Conference: 10th World Biomaterials Congress, Montréal, Canada, 17 May - 22 May, 2016. Presentation Type: Poster Topic: Biomaterials in mesenchymal and hematopoietic stem cell biology Citation: Muller S, Nicholson L, De Havilland J, Dalgarno K, Dickinson A and Wang X (2016). Effect of apatite-wollastonite glass ceramics on the migration of mesenchymal stem cells. Front. Bioeng. Biotechnol. Conference Abstract: 10th World Biomaterials Congress. doi: 10.3389/conf.FBIOE.2016.01.01525 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 27 Mar 2016; Published Online: 30 Mar 2016. Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Sylvia Muller Lindsey Nicholson Julian De Havilland Kenny Dalgarno Anne Dickinson Xiao-Nong Wang Google Sylvia Muller Lindsey Nicholson Julian De Havilland Kenny Dalgarno Anne Dickinson Xiao-Nong Wang Google Scholar Sylvia Muller Lindsey Nicholson Julian De Havilland Kenny Dalgarno Anne Dickinson Xiao-Nong Wang PubMed Sylvia Muller Lindsey Nicholson Julian De Havilland Kenny Dalgarno Anne Dickinson Xiao-Nong Wang Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.