Effect of antioxidants supplementation on the quality of Beetal buck semen stored at 4°C.

A S Yadav,Pardeep Singh,Anuradha Kumari,Meenakshi Virmani,A P Rath,Archana Sarangi,Subhasish Sahu,H M Ajithakumar

doi:10.14202/vetworld.2017.1184-1188

Abstract

Aim:An experiment was designed to evaluate the role of Vitamin E and glutathione in improving the seminal parameters during hypothermic storage of liquid semen at 4°C for 72 h.Materials and Methods:Thirty-six semen ejaculates were collected by artificial vagina from 6 bucks (Beetal) during the normal reproduction season (September to November) at weekly interval. The samples were centrifuged, and the seminal plasma was removed. The sperm pellet was diluted with Tris-based extender and divided into three groups. Group T1: Control samples without antioxidants, Group T2: Samples supplemented with tocopherol at 3 mM, and Group T3: Samples supplemented with glutathione at 1 mM. The samples were evaluated for progressive motility, percent liveability, percent abnormal spermatozoa, and acrosome integrity after liquid preservation for 0, 24, 48, and 72 h. The level of lipid peroxidation and antioxidant enzymes, namely, glutathione peroxidase (GPx) and superoxide dismutase (SOD) were estimated after liquid preservation for 0 and 72 h.Results:It was observed that, after storage of semen at 4°C up to 72 h, the progressive sperm motility, percent liveability, percent abnormal spermatozoa, and percent intact acrosomes were significantly (p<0.05) higher in group T2 and T3 samples as compared to control. However, the level of lipid peroxidation in T2 and T3 groups was significantly (p<0.05) lower after 72 h of incubation at 4°C. Similarly, GPx and SOD values were significantly (p<0.05) increased in T2 and T3 groups after 72 h of storage at 4°C.Conclusion:Thus, it can be concluded that Vitamin E and glutathione supplementation at 3 mM and 1 mM, respectively, while preserving the semen samples at 4°C helped in maintaining the seminal parameters up to 72 h and protected the spermatozoa from oxidative damage.

Highlights

Goat husbandry is the backbone of rural economy in India as it helps to sustain the livelihood of rural poor because of providing meat, milk, skin, hair, and droppings
Conclusion: it can be concluded that Vitamin E and glutathione supplementation at 3 mM and 1 mM, respectively, while preserving the semen samples at 4°C helped in maintaining the seminal parameters up to 72 h and protected the spermatozoa from oxidative damage
The percent progressive sperm motility, percent of live sperm, percent of abnormal spermatozoa, and percent intact acrosome in liquid-preserved semen of Beetal bucks in control and both the treatment, i.e. after addition of Vitamin-E and glutathione at 4°C after 0, 24, 48, and 72 h of incubation are presented in Table-1

Summary

Introduction

Goat husbandry is the backbone of rural economy in India as it helps to sustain the livelihood of rural poor because of providing meat, milk, skin, hair, and droppings. Goat meat (Chevon) is most preferred and widely consumed meat in the country, and goat milk has traditionally been known for its medicinal properties, and it’s all round health-promoting traits [1]. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated

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