Effect of Antioxidant Preservative on Cold Protection Ability of Low Grade Riverine Buffalo (Bubalus bubalis) Bull Spermatozoa

P K Pankaj,V S Raina,A Mishra,B Roy,T K Mohanty

doi:10.5713/ajas.2009.70267

Abstract

An experiment was conducted to investigate the effect of Butylated Hydroxy Anisole (BHA), Butylated Hydroxy Toluene (BHT), Pentoxifylline (PTX), Theophylline (TPY) and Theobromine (TBR) on cold protection ability of Murrah buffalo semen at room (22-25°C) and refrigerated temperature (4-7°C). Each semen sample was divided into six parts of equal volume and sperm concentration; the first was kept as a control and the remaining five were treated with BHA, BHT, PTX, TPY or TBR. Sperm motility, abnormal spermatozoa, live-dead count, hypo-osmotic swelling and acrosomal integrity were studied at room and refrigerated temperature for various incubation periods viz.; 0, 4, 8, 12 and 24 h at room and 0, 12, 24, 36, 48, 60 and 72 h at refrigerated temperature. Significant improvement in sperm motility, live-dead count, hypo-osmotic swelling and acrosomal integrity were observed in BHT, PTX and TPY fortified extender at room and refrigerated temperature for various incubation periods. From the present study it could be concluded that cold protection ability of buffalo semen can be improved through the addition of BHT followed by PTX and TPY.

Highlights

Buffaloes in the Asian continent play an important role as a producer of milk, draught power, dung and other value added products
A deficiency of these fractions can affect the overall protection of the spermatozoa from oxidative damage, which can have a negative effect on sperm motility and fertilization
Overall the results indicated that Plant Based Extender (PBE) fortified with Butylated hydroxy toluene (BHT), PTX and TPY could support the preservation of livability of spermatozoa to the maximum extent in storage at refrigerator temperature (4 to 7°C)

Summary

Introduction

Buffaloes in the Asian continent play an important role as a producer of milk, draught power, dung and other value added products. PTX increases cAMP level by a methylxanthine inhibition of phosphodiesterase and improves motility, capacitation and acrosome reaction (Yovich et al, 1984; Sikka et al, 1995). This increase in cAMP causes activation of protein kinase and phosphorylation of endogenous protein. Motility of spermatozoa is increased due to utilization of increased energy production by accelerating glycolysis and TCA cycle activity (Haesungcharern and Chulavatnatol, 1973)

Methods

Results

Conclusion