Abstract
The problem of multidrug-resistant pathogens as bacteria, fungi and yeast in addition to the restriction of using antibiotics as growth promoting substances in feed has attracted attention for looking for alternative sources instead of conventional antibiotics like natural products which have antimicrobial activity. Much interest and researches have been focused on using natural antimicrobial peptides and chemicals extracted from animal secretions and some insect’s venoms as they exhibit antimicrobial activity against pathogens with lower resistance and higher synergistic effects if they were given in combination with blends of them. In this paper, some antibacterial peptides extracted from honeybees venom and expression with yeast Saccharomyces cerevisiae will be discussed as well as antimicrobial chemicals extracted from giraffes in addition to their inhibitory effects on liver microsomal enzymes CYP450 will be discussed also with its activity against microorganisms which are Agrobacterium rhizogenes, Aspergillus niger, Candida albicans and E. coli using optical density analysis technique then their minimum inhibitory concentrations (MIC) will be determined as well as ICs 50 to measure the potency to inhibit a biological function using programmes like Gene5, graph pad prism and clone manager as well as testing antimicrobial activity of some chemicals which are provided in animal secretions.
Highlights
MethodsThe typical parameters used for fluorescent substrates using microsomal P450s is by adjusting conditions’ assays per 100 μL volumes, black 96 well plates and 100 mM potassium phosphate buffer with PH 7.4
Much interest and researches have been focused on using natural antimicrobial peptides and chemicals extracted from animal secretions and some insect’s venoms as they exhibit antimicrobial activity against pathogens with lower resistance and higher synergistic effects if they were given in combination with blends of them
Some antibacterial peptides extracted from honeybees venom and expression with yeast Saccharomyces cerevisiae will be discussed as well as antimicrobial chemicals extracted from giraffes in addition to their inhibitory effects on liver microsomal enzymes CYP450 will be discussed with its activity against microorganisms which are Agrobacterium rhizogenes, Aspergillus niger, Candida albicans and E. coli using optical density analysis technique their minimum inhibitory concentrations (MIC) will be determined as well as ICs 50 to measure the potency to inhibit a biological function using programmes like Gene5, graph pad prism and clone manager as well as testing antimicrobial activity of some chemicals which are provided in animal secretions
Summary
The typical parameters used for fluorescent substrates using microsomal P450s is by adjusting conditions’ assays per 100 μL volumes, black 96 well plates and 100 mM potassium phosphate buffer with PH 7.4. Regenerating system is by frozen solution A and solution B. Regenerating system solution A stock is held by 65.42 mM magnesium chloride solution containing 26.13 mM NADP+ and 65.77 mM of glucose-6phosphate. In each well NADP+ 1.3 mM, glucose-6-phosphate 3.3 mM in magnesium chloride buffer 3.3 mM. Regenerating system solution B stock 5 mM tribasic sodium citrate contains 40 units per ml of glucose-6-phosphate dehydrogenase. In each well 0.04 units of glucose-6-phosphate dehydrogenase in buffer sodium citrate 50 μM
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
