EFFECT OF ANTIMICROBIAL AGENTS ON THE BIOFILM GROWTH OF CLINICAL ISOLATES

Abstract

OBJECTIVE. To study the effect of active extracellular substances of lactobacilli and antimicrobial agents on the inhibition and destruction of the biofilms formed clinically relevant microorganism strains. MATERIALS AND METHODS. The study of the impact of different agents on the biofilmformation and growth was carried out on resistant clinical strains of microorganisms obtainedfrom patients with post-surgical infectious inflammatory complications. We used wound dressing solution, cutaneous antiseptic, filtrates of 19 clinical strains of lactobacilli and a strain of Lactobacillus plantarum from the probiotic «Lactobacterin dry» (Microgen Nizhny Novgorod, series 46 / 06-1209) as a reference strain-producer of bacteriocins for biofilm inhibition. Biofilms were incubated for 48 hours on glass carriers at 37°C and visualized with a light microscope at 960x magnifying. RESULTS. All substances possess a good inhibitory potential and have approximately same level of effect. The skin antiseptic and wound washing fluid have only an inhibiting effect on the biofilmformation process, while the having a bactericidal effect on plantonicform of the cells. The lactobacilli filtrate inhibited the biofilmformation and was also able to destroy preformed 24-hour bacterial films. CONCLUSION. The use of lactobacilli bacteriocins can reveal additional opportunities for combating the infection associated with biofilm forming microorganisms.