Effect of antibacterials on surface properties and adhesion to uroepithelial cells in Klebsiella pneumoniae

Abstract

The effect of sub-inhibitory and inhibitory concentrations of antimicrobials including aminoglycosides, third generation cephalosporins and quinolones on the surface properties and adhesion of Klebsiella pneumoniae to uroepithelial cells (UECs) was examined. Antibiotics, ceftazidime and ofloxacin at 1/4, 1/8 A— MIC and minimum inhibitory concentration (MIC) induced filament formation in bacteria, however cells treated with amikacin were similar in length to control organisms but showed a rough topology under the scanning electron microscope. An increase in bacterial hydrophobicity and decrease in uronic acid content were noted in the presence of ceftazidime and ofloxacin at MIC and sub-MIC level. However, amikacin at MIC level caused decreased hydrophobicity of the cells and the uronic content remained the same. This study clearly indicates that, although ceftazidime and ofloxacin brought about profound changes in cell surface characteristics, these changes did not result in any advantage to the bacterial cell in terms of adhesion. In contrast, with amikacin, which did not show any appreciable change in cell morphology or surface topology, exposure markedly increased the adherence of bacteria to UECs, indicating that the prophylactic use of this antibiotic not only induces resistance in bacteria but can also promote the colonization of UECs.