Abstract
During anaphylaxis, histamine (His) and SRA-A are the most important chemical mediators. In this paper, a tissue bioassay system was contrived for simultaneous determination of His and SRS-A released from guinea-pig lung during anaphylaxis. The system was composed of two guinea-pig ileums superfused with Krebs Henseleit solution (K.H.sol.) kept at 37°C and gassed with the carbogen. The upper ileum was treated with atropine, 1 μg/ml, alone and the lower one was treated with atropine,1 μg/ml,and chlorpheniramine,1 μg/ml. The guinea-pig lung was incubated for 4 hr in K.H.s0i. containing 4% serum prepared from rabbit sensitized with ovalbumin. The releasing amount of chemical mediators from 1-week-old guinea-pig was much more than that from 6-week or 12-week-old one. The contraction of lower ileum which was markedly inhibited by FPL-55712 was potentiated by pretreatment with indomethacin, 1 μg/ml. As a result, the chemical mediators from guinea-pig lung might be mainly His and SRS-A. Isoproterenol (Iso), salbutamol Sal) andNAB-365 (β2-adrenoceptor stimulant) inhibited the release of His and SRS-A nearly 1000 times as much as theophylline or disodium cromoglycate. As the inhibitory effects of Iso, Sal and NAB-365 were antagonized by propranolol, 3 μg/ml, mediator-release during anaphylaxis can be considered to be inhibited via β- adrenoceptors.
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