Abstract

The experiment was carried out in attempt to develop an efficient and simple protocol for diploidization of gynogenic onion ( Allium cepa L.) embryos. Well-developed embryos derived from flower bud cultures of different onion materials were used. Doubling took place in vitro, on two regeneration media supplemented with one of four anti-mitotic agents, i.e. trifluralin, oryzalin, amiprophos-methyl (APM) or colchicine in various concentrations. Embryos were plated on the media for 24 or 72 h and then transferred onto regeneration media without anti-microtubule compounds. Frequency of developing embryos ranged from 45 to 77% and was similar for both time regimes. In most combinations doubling substances caused some vitrification of developing embryos, on average 11%. Among the evaluated regenerants 19–35% were diploids. The highest doubling efficiency was achieved for embryos cultured on media supplemented with 50 μM trifluralin or oryzalin or APM. Since APM induced the least vitrification of the onion tissue it may be proposed as the best chromosome doubling compound for Allium cepa.

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