Effect of anti-CIRP antibody on inflammatory response, tumor formation and abdominal aortic aneurysm in rats

Yuqing Wang,Lantao Lu,Shuntong Gu,Weiyan Li

doi:10.4314/tjpr.v19i6.15

Abstract

Purpose: To investigate the effect of anti-cold induced RNA binding protein (CIRP) antibody on inflammation, tumor formation and abdominal aortic aneurysm in rats.Methods: Thirty healthy male Wistar rats were assigned to pseudo-operation, abdominal aortic aneurysm model, and anti-CIRP groups, with 10 in each group. The levels of CIRP, TNF- α, monocyte giant cytokine chemokine-1 (MCP-1), Toll-like receptor 4 (TLR4)) and nuclear factor kappaB (NF- κB)were determined compared among the groups.Results: At both 2 and 4 weeks, the expression of CIRP protein in the model group was significantly higher than that in the sham operation group (p < 0.05). At these two time-points, tumor formation and maximum diameter were higher in anti-CIRP and model control rats than in pseudo-operation rats. After 4 weeks of treatment, the protein expressions of TNF- α, MCP-1, TLR4 and NF-κB were higher in anti-CIRP and model control rats than in pseudo-operation rats, but were lower than model control values (p < 0.05).Conclusion: CIRP expression is significantly increased in abdominal aortic aneurysm tissue and serum, and is involved in the onset and progress of abdominal aortic aneurysm. Anti-CIRP antibody therapy effectively suppresses tumorigenesis, and inhibits tumor wall inflammatory reaction viaTLR4/NF-κB pathway. This finding provides a clue and new strategy for the clinical management of abdominal aortic aneurysm. Keywords: CIRP, Abdominal aortic tumor wall, Inflammatory reaction, Protein expression, Tumor body

Highlights

Abdominal aortic aneurysm refers to an arterial degenerative disease in which the abdominal aortic diameter is increased by > 50 % [1]
Protein expressions of coldinduced RNA binding protein (CIRP), TNF-α, MCP-1, Toll-like receptor (TLR4) and Nuclear transcription factor kappa B (NF-κB) in tumor wall tissues of rats in each group were assayed with western blotting: First, the tumor wall tissues of each group were collected, and the total cell protein was extracted by adding protein lysate, and the BCA kit was used to determine and quantify the protein concentration
The results showed that % tumor formation and maximum diameter were markedly higher in anti-CIRP and model control rats than in pseudo-operation rats, and the maximum diameter and % tumor formation in the anti-CIRP group were significantly lower than those of the model control group

Summary

INTRODUCTION

Abdominal aortic aneurysm refers to an arterial degenerative disease in which the abdominal aortic diameter is increased by > 50 % [1]. The rats were randomly divided into sham operation group (n = 10) and abdominal aortic aneurysm model group (n = 20). Protein expressions of CIRP, TNF-α, MCP-1, Toll-like receptor (TLR4) and Nuclear transcription factor kappa B (NF-κB) in tumor wall tissues of rats in each group were assayed with western blotting: First, the tumor wall tissues of each group were collected, and the total cell protein was extracted by adding protein lysate, and the BCA kit was used to determine and quantify the protein concentration. The RT-PCR method was used to determine CIRP mRNA expression in the tumor wall of rats. The tumor wall tissues of rats in each group were taken and the total RNA was extracted according to the RNA extraction instructions.

RESULTS

DISCUSSION

CONCLUSION

Conflict of interest