Effect of Anthocyanin on the Ovarian Damage and Oxidative Stress Markers in Adult Female Rat After Torsion/Detorsion

Abstract

Objectives: This experimental study aimed to explore the impact of anthocyanin on histopathological alterations and oxidative stress induced by ovarian torsion/detorsion in mature rats. Materials and Methods: A total of 32 female Wistar rats were categorized into four groups: G1 (sham), G2 (ovarian torsion endured for 3 hours followed by detorsion), G3 (ovarian torsion persisted for 3 hours with prior administration of 100 mg/kg anthocyanin followed by detorsion), and G4 (received 100 mg/kg anthocyanin). After a reperfusion interval of 10 days, blood specimens were obtained for quantifying estrogen, testosterone, and oxidative stress markers in the plasma. Histomorphometric analysis was done to evaluate post-procedural tissue alterations. Results: The histopathological observations revealed considerable degenerative modifications in the G2 group (subjected to torsion/ detorsion) compared to the other groups (P<0.001). Estrogen, glutathione peroxidase (GPX), and superoxide dismutase (SOD) levels displayed notable reduction in the G2 group; however, these levels were reinstated in the anthocyanin-treated groups. The level of malondialdehyde (MDA), an indicator of oxidative stress, surged during the ischemic phase (P<0.001). Conclusions: Administration of anthocyanin led to a decline in MDA levels across the other groups. These findings collectively suggest that anthocyanin supplementation holds the potential to shield ovarian tissue against oxidative stress and structural damage incurred as a consequence of torsion/detorsion events.