Abstract
Context: Furosemide is an anionic molecule and has very low absorption in gastro intestinal tract.Objective: The aim of this study was to investigate the effect of anionic macromolecules on the intestinal permeability of Furosemide.Materials and methods: The intestinal permeability of Furosemide was determined using single-pass intestinal perfusion technique in rats. Briefly a jejunal segment of ∼10 cm was isolated and cannulated in both ends for inlet and outlet solution. The perfusate was collected every 10 min and samples were analyzed using the RP-HPLC method. Test samples containing furosemide and two anionic macromolecules, sodium carboxy methyl cellulose and sodium alginate, at different concentrations were used.Results: The obtained data showed that existence of Sodium carboxy methyl cellulose significantly increased the Peff values in all three investigated concentrations (p < 0.05) but sodium alginate only in concentrations <0.1% increased drug permeability.Discussion: It is concluded that the anionic macromolecules at specific concentrations could alter the permeability of anionic drugs across the biological membranes.Conclusions: Donnan phenomenon and chelating property of macromolecules could be attributed to the observed effect.
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