Effect of angiotensin invertin inhibitor and Aldosterone antagonists on vascular cell adhesion molecule-1 in adriamycin-induced nephrotic rats

Abstract

Objectives To observe the bettering effect of Benazepril and Spironolactone on renal tubule mesenchyme damage in adriamycin ( ADR)-induced nephrotic rat models. Methods Among 42 cleaned male Sprague-Dawley (SD) rate, 7 were randomly taken as normal control group (A group ). And the rest 35 rats were induced by tail intravenous injection of ADR( 1.75mg/kg) . twenty-nine ADR-induced nephrotic male Sdrate were randomly divided into model control group(B group) , Benazepril treatment group(C group) , Spironolactone treatment group( D group) , and Benazepril-Spironolactone-combined treatment group(E group). At the end of 6,12, and 18 weeks, the samples of 24h urine were collected. And at the end of the 18th week, the rats were executed, and blood specimen was collected to be detected. Nephridial tissue was taken and pathomorphological observation was carrying out. Meanwhile, the expression of renal tubule mesenchyme VCAM-1 was detected using immunohisto chemistry technique. Results Compared with B group, serum potassium of rate in D and E group increased subtlely. In C, D, and E group, the levels of serum VCAM-1 x cholesterol and triglycerides all decreased, the proteinuria also decreased, while the levels of serum albumin increased. And the joint treatment had a more obvious effect.Conclusions Combined using Benazepril and Spironolactone can decrease the levels of serum VCAM-1and urine protein, but it also has the risk of serum potassium increasing. And it has a protecting role for renal tubule mesenchyme damage in adriamycin (ADR)-induced nephrotic rate. Key words: Angiotnsin-Converting Enzyme Inhibitors