Effect of angiotensin(1-7) on the expression of E-selectin induced by angiotensinII and its mechanism in vascular endothelial cell

Abstract

Objective. To evaluate the effects of angiotensin II on the expression of tissue factor (TF) in the cell line ECV304 and the effects of angiotensin (1-7) on induced TF angiotensin II expression. Methods. ECV304 cells were cultured in RPMI1640 medium. The procoagulant activity (PCA) was determined with one-stage clotting assay and TF mRNA examined by semi-quantitative RT-PCR. Immunohistochemical analysis was performed to evaluate the activation of NF-κB. Results. Compared with the control, angiotensin II (10 –10 – 10 –6 mol/L) significantly increased PCA of ECV304 cells and TF mRNA expression in a dose-dependent manner (P < 0.01, r = 0.9631) and the peak concentration was 10 –6 mol/L. Angiotensin (1-7) (10 –10 – 10 –7 mol/L) alone could not change the PCA, but pretreated the ECV304 cells with angiotensin (1-7), which