Effect of an Introgressed Nicotiana tomentosa Leaf Number QTL on Yield and Quality Characteristics in Flue‐Cured Tobacco

Abstract

ABSTRACTIncreased cured leaf yield is an important breeding target for flue‐cured tobacco (Nicotiana tabacum L.), but genetic improvement for this trait has slowed in the last thirty years. Yield is positively correlated with days to flowering and leaf number in this species. Use of novel allelic diversity influencing these traits might contribute to further yield improvement. In this study, an introgressed quantitative trait locus (QTL) affecting flowering time and leaf number, designated as Many Leaves (Ml), was backcrossed into three genetic backgrounds of flue‐cured tobacco. Field evaluation of nearly isogenic MlMl lines and Mlml hybrids indicated that the QTL acted in an additive to partially dominant fashion to delay flowering and increase leaf number. This resulted in significant increases in yield and cash return per hectare. As the zygosity of Ml increased, corresponding decreases in percentage total alkaloids and increases in percentage reducing sugars were observed, however. Incorporation of Ml into flue‐cured tobacco breeding programs may be useful for increasing cured leaf yields in certain environments. Concurrent modification of the genetic potential to accumulate alkaloids would be also important under current industry requirements, however.