Effect of an anti-inflammation treatment on ligature-induced experimental periodontitis

Abstract

Abstract Objective Inflammation-related immune responses and bone metabolism lead to extensive tooth loss in periodontitis. This study is to investigate the effect of anti-inflammation treatment in vitro and on ligature-induced experimental periodontitis in vivo. Methods Splenocytes were isolated from C57BL/6J mice and cultured for 48 hours under the following conditions: control, lipopolysaccharide (LPS) (1 μg/ml), LPS(1 μg/ml)+fenofibrate (1, 10, 50, 100 μM). The mRNA level and secreted protein level of TNF-α expression were detected by RT-qPCR and ELISA respectively. Silk ligatues (7–0) were tied around both maxillary second molars of C57BL/6J mice for two weeks. Optimized dose of fenofibrate (100 μM) and vehicle control were injected into contralateral side of palatal gingiva on days 3, 6 and 9. The palatal gingival tissues and maxillary bone were collected on day 14 to determine expressions of TNF-α, IL1, RANKL/OPG and periodontal bone resorption respectively. Results TNF-α expressions of cultured spleen B cells were significantly increased in the presence of LPS when compared with control group, and significantly reduced by fenofibrate treatment in a dose-dependent manner from (1–100 μM). Gingival mRNA levels of TNF-α, IL1 and the ratio of RANKL/OPG were significantly decreased after injection of fenofibrate, when compared to the control side (p<0.05). The periodontal bone loss were significantly decreased on the side with injection of fenofibrate, as compared to the control side (p<0.05). Conclusion An anti-inflammation treatment, fenofibrate, inhibited the inflammation and periodontal bone loss in ligature-induced experimental periodontitis.