Effect of Ampicillin wastewater on the T-AOC and LDH activities of Zebrafish

Abstract

In order to define eco-toxicity effect of Ampicillin wastewater on zebrafish, the indoor exposure method was used to study the impact of Ampicillin wastewater on zebrafish. In this study, zebrafish was exposed to ampicillin wastewater of 0.5%, 1.0%, 1.5% and 2.0% groups for 15 days to study the effect of T-AOC (Total Antioxidative Capacity) and LDH (Lactate Dehydrogenase) activities. According to the experimental data, the T-AOC and LDH activities in zebrafish muscle tissue had changed significantly during the period of exposure. The experimental results showed that the T-AOC value of zebrafish in each exposed group showed a “Λ” type change trend. On the 6th day, the 2.0% exposed group reached the maximum value of the whole test period (6.5399U•mg−1). The change of LDH activity was similar to that of T-AOC activity, showing a trend of “sharp increase--decline”. Among them, on the 12th day, the 1% exposed group reached the maximum (792.28 U•mg−1). The research shows ampicillin wastewater can cause some degree of oxidative damage to zebrafish.