Effect of ammonia-N on histology and expression of immunoglobulin M and component C3 in the spleen and head kidney of Pelteobagrus vachellii

Abstract

Ammonia-N is toxic to many aquatic animals and serves as a key stress factor in aquatic environments. The effects of ammonia-N stress on the immune response of darkbarbel catfish Pelteobagrus vachellii were investigated in this study. Changes in overall histology, and in the expression of complement C3 and immunoglobulin M (IgM) in spleen and head kidney, and lysozyme and C3 in serum, were measured in 1 and 5mg/L ammonia-N. Hyperemia, melano-macrophage assembly and loose splenosis were evident in spleen tissue. Both lysozyme and component C3 were significantly reduced in serum (P<0.05) under both stress treatments, although lysozyme was increased slightly at 24h in the 1mg/L treatment. C3 mRNA expression increased at 6h in spleen and 6–12h in head kidney then decreased rapidly (P<0.05), although C3 mRNA recovered to control levels in spleen at 96h after the 1mg/L treatment (P>0.05). IgM expression also increased significantly at 6–12h in spleen and 6–24h in head kidney after the 1mg/L treatment (P<0.05). A similar overall pattern were observed with 5mg/L ammonia (P<0.05); IgM mRNA expression was elevated at 6h in spleen and 6–12h in head kidney (P<0.05) then decreased to levels below controls (P<0.05). These results suggest exposure to 5mg/L ammonia-N could damage the histological structure of spleen, diminish lysozyme and component C3 serum content, and suppress C3 and IgM expression in spleen and head kidney.