Effect of Amino Acids on Secondary Somatic Embryogenesis of Moroccan Cork Oak (&amp;lt;i&amp;gt;Quercus suber&amp;lt;/i&amp;gt; L.) Tree

Safaâ Rahmouni,Patrick Martin,Alain Badoc,Ahmed Lamarti,Zineb Nejjar El Ansari,Mohammed L’Bachir El Kbiach

doi:10.4236/ajps.2020.115047

Effect of Amino Acids on Secondary Somatic Embryogenesis of Moroccan Cork Oak (&lt;i&gt;Quercus suber&lt;/i&gt; L.) Tree

Safaâ Rahmouni, Patrick Martin + Show 4 more

Open Access

https://doi.org/10.4236/ajps.2020.115047

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Abstract

In the present study, we tested the effect of amino acids on secondary somatic embryogenesis of Moroccan cork oak (Quercus suber L.). Secondary mature and immature somatic embryos were obtained from primary somatic embryos cultured in N30K medium supplemented with nineteen amino acids. Stimulation of embryogenesis was dependent on the type and concentration of amino acid in the medium. Thus, L-glutamine and L-asparagine at 3.42 mM have been proved to be the most favorable for the formation of functional somatic embryos and the induction of secondary somatic embryos.

Highlights

Mediterranean forests have been extensively exploited for their different resources
We tested the effect of amino acids on secondary somatic embryogenesis of Moroccan cork oak (Quercus suber L.)
Secondary somatic embryogenesis is an effective tool for large-scale multiplication of cork oak in particular and for woody plants in general

Summary

Introduction

Mediterranean forests have been extensively exploited for their different resources. These forests cover 85 million hectares, representing about two percent of the world’s forest area (4033 million hectares) [1] and contain more than 25,000 plant species, especially conifers and hardwood species, like oaks [2].Cork oak (Quercus suber L.), a species belonging to the Fagaceae family, is one of the most characteristic oak species of the Mediterranean ecosystem. Mediterranean forests have been extensively exploited for their different resources. These forests cover 85 million hectares, representing about two percent of the world’s forest area (4033 million hectares) [1] and contain more than 25,000 plant species, especially conifers and hardwood species, like oaks [2]. Cork oak (Quercus suber L.), a species belonging to the Fagaceae family, is one of the most characteristic oak species of the Mediterranean ecosystem. It covers 2.2 million hectares of the world forest surface and plays important ecological, economic and social roles. It belongs to the section Suber of the subgenus Cerris [5]. It is extremely polymorphic, distributed in the Western Mediterranean region, in the various European and African countries and territories: Portugal, Spain, Southern France, Morocco, Algeria, Tunisia, Corsica, Sardinia, Italy and Slovenia [6]

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