Effect of aminalon and picamilon on NLRP3 inflammasome activation in pancreatic cells during alloxan-induced diabetes mellitus

Abstract

It has been suggested that NLRP3 (NOD-, LRR- and pyrin domain-containing protein 3) activation is a critical trigger for functional impairment and damage to pancreatic -cells in type 2 diabetes mellitus (DM). In this work, using the immunohistochemical method, we studied the effect of aminalon and picamilon on the early modulation of NLRP3 inflammasome activity during experimental alloxan-induced DM in laboratory rats. Per os administration of aminalon (500 mg/kg) and picamilon (250 mg/kg) for 5 days before the administration of alloxan and for 2 days after the injection, suppression of NLRP3 activity was noted, as indicated by a significant decrease in the area of immunopositive pancreatocytes to (21,30 5,44) and (39,31 5,24) %, respectively, relative to the value in the group of animals that were not treated (75,197,69%). The results of the study showed that the studied preparations with GABAergic action promoted correction of functional disorders of the pancreas during alloxan-induced DM by inhibiting activation of NLRP3 inflammasome in pancreatocytes.