Effect Of Alpha-Tocopherol On Spermatozoa Death Of Apoptosis and Necrosis in Rats (Rattus Norvegicus) Exposed 2,3,7,8- Tetrachlorodibenzo-P-Dioxin

Valderama Gomang

doi:10.20473/jscrte.v5i1.29381

Abstract

The aims of this research was to investigate effect of alpha-tocopherol on spermatozoa death in form of apoptosis and necrosis in rats (Rattus norvegicus) exposed 2,3,7,8- tetrachlorodibenzo-p-dioxin. Male rats were administered TCDD and alpha-tocopherol in experimental groups. Five experimental groups of a combination of TCDD and alpha- tocopherol were designed as follows; 0.5 ml of corn oil (control negative group, K-), 700 ng/kg/d of TCDD and 0.5 ml of corn oil (treatment control), 700 ng/kg/d of TCDD and 77 ng/kg/d of alpha-tocopherol (Group P1), 700 ng/kg/d of TCDD and 140 mg/kg/d of alpha-tocopherol (Group P2), 700 ng/kg/d of TCDD and 259 mg/kg/d of alpha- tocopherol (Group P3) respectively. Alpha-tocopherol and TCDD were given by oral gavage for 20 days. The result indicated that TCDD increased spermatozoa death in form of apoptosis and also necrosis. Alpha-tocopherol at 259 mg/kg/d most effective to decreased spermatozoa death number. The conclusion indicated that alpha-tocopherol at 259 mg/kg/d effective to decreased the spermatozoa death in form of apoptosis and necrosis in rats (Rattus norvegicus) exposed 2,3,7,8-tetrachlorodibenzo-p-dioxin.

Highlights

Environmental pollution is an event that spreads certain levels of chemicals that can disrupt the balance of the environment
Conditions due to exposure that occurred showed an increase in spermatozoa morphological abnormalities, decreased motility, and decreased oocyte penetration ability in men exposed to dioxin during the perinatal and lactation periods, as well as in men exposed in adulthood
Dioxin is a toxic compound that belongs to the group of environmental contaminants which are mainly produced through industrial processes such as burning plastic waste, power plants, and making herbicides and pesticides (Procopec et al, 2013; Ribeiro et al, 2019)

Summary

INTRODUCTION

Environmental pollution is an event that spreads certain levels of chemicals that can disrupt the balance of the environment. Dioxin is a toxic compound that belongs to the group of environmental contaminants which are mainly produced through industrial processes such as burning plastic waste, power plants, and making herbicides and pesticides (Procopec et al, 2013; Ribeiro et al, 2019). Exposure to dioxin can accumulate in human foodstuffs such as milk and dairy products, meat fat tissue, chicken eggs, and the process of burning waste, especially those made from plastic (Ciftci et al, 2010). As a result of the decrease in FSH hormone secretion, it causes a decrease in the number of Sertoli cells which function to nourish spermatogenic cells during the period of spermatogenesis. The decrease in the work activity of reproductive hormones will disrupt the process of spermatogenesis which is one of the consequences of the mechanism of death of spermatozoa. Vitamin E is divided into several species and the one that has the best antioxidant ability is the alpha-tocopherol group (Uzunhisaarcikli and Calendar., 2011)

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CONCLUSION