Effect of Allogenic Omental Graft on Esophegotomy Incision in Dogs Models

Abstract

The study aimed to evaluate the beneficial effect of allogenic omental graft on esophageal healing in dogs.Twenty healthy male adult dogs were used they were randomly divided into two equal groups (controlgroup 10, treated group 10). Under aseptic technique 4 cm skin incision was made in the midline of thecervical(ventral) of animal by scalpel, after that the incision was sutured immediately by polyglycolic acidsuture materials 0.3 USP used simple continous technique, and the omental graft extracted from same animalsby laprotomy piece of greater omentum was then placed over the oesophagotomy incision of the experimentgroup and secured in place with tacks sutures, In the control group, the esophagotomy suture line was leftwithout graft. Esophageal healing process follow up 1,2 and 3 weeks post operation by histopathologicalexamination, the result display in treated group on the first week post operation development of collagen andlamina properia contain congested blood vessels with epithelial regeneration. 2 weeks post operation showthe epithelium of the mucosa of the esophagus was formed by hyperplasia of squamous cells. The surfaceof these epithelia was covered by keratin with present of bundle of collagen and fibroblasts. 3 weeks postoperation showed the epithelium of esophagus was covered by keratin, with the presence of blood capillariesand also, there was lymphocytic infiltration in the lamina properia with RBC. The tunica muscularis wasconsisting of skeletal muscle fibers, inner longitudinal, and outer circular, proliferation of fibroblast withdeposition of collagen, while in control group histopathological examination in the first week show Theepithelium of the esophagus was stratified squamous with the presence of keratin on its surface, 2 weekspost operation show The mucosa of the esophagus consisted of stratified squamous epithelium with a keratinlayer on its surface. The lamina properia was composed of loose connective tissue with infiltration of manyWBC and blood vessels, 3 weeks post operation show the basal layer of epithelium had many folds whichare interdigitation with the underlying lamina properia, which appeared loose connective tissue with fewnumbers of fibroblasts and lymphocytes, the healing of treated group better than control group.